The success of your PCR reaction depends on the quality of the starting materials. Accuris dNTP’s are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.
Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.
The 40mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10mM each. The 100mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.
Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.
These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range. An inert blue dye is also mixed into the qMAX Green to allow easy visualization during pipetting.
Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.
Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.
All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.
Three versions of our One Step qPCR kits are available.
The Accuris cDNA Synthesis Kit is a 2-tube format for easy reaction set up and is ideal for 4pg to 0.5µg of input RNA. One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a nonbiased population of cDNA.
Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays. qMax Probe utilizes Accuris Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.
qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments. To check compatibility with a specific instrument, visit www.accurisusa.com/PCRselector.
Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.
The AutoMATE™ 96 from Accuris Instruments is a fully automated, high throughput liquid handling workstation that improves productivity when filling microplates. Capable of dispensing up to 96 channels simultaneously, the AutoMATE 96 significantly reduces the processing time and errors associated with manual pipetting.
The included tablet features an intuitive app for programming and operation. Unlike many 96-well pipetting stations, the AutoMATE 96 is “hands-free”, with motorized tip loading/ejection and a four position, automated, sliding platform that precisely aligns the tips with the appropriate microplate/reservoir. Once programmed, the instrument can run protocols to completion without any manual positioning or adjustment.
Three interchangeable heads allow for a pipetting range from 1.0µL to 1000µL. The AutoMATE 96 is compact enough to fit onto a standard lab bench or into a biosafety cabinet.
Standard Pipetting: Aspirating and dispensing reagents by hand is labor-intensive and error prone. With the AutoMATE 96, reagents can be aspirated and dispensed automatically, ensuring accurate and precise transfer to and from microplates or reservoirs.
Multi-Dispensing (Repeat Pipetting): Continually filling microplates is a time-consuming process that can often result in hand fatigue. With the Multi-Dispensing function, repeatedly filling microplates can be automated and accomplished quickly without introducing hand strain or fatigue.
Secondary-Dispensing (Plate Filling): Many protocols and applications call for filling a plate with multiple samples or reagents. With the AutoMATE 96, multiple reagents can be aspirated and dispensed into the same microplate. For improved results, each dispense can be programmed to be include a mixing step.
Serial Dilutions: Performing serial dilutions via manual methods is labor-intensive and error prone. Errors in this process will amplify subsequent dilutions as the process continues. With the AutoMATE 96, serial dilutions can be performed automatically and error free.
Mixing: Mixing reagents by hand is strenuous and can be inconsistent. With the AutoMATE 96, mixing can be performed automatically with adjustable speeds, ensuring consistency.
Reverse Pipetting: When the accuracy of pipetting volume is critical, the AutoMATE 96 can be set to reverse pipetting mode. This technique greatly improves accuracy &precision with small volumesof biological fluids.
Two operating modes are available and can be selected per the user’s preference.
Manual Mode: Perform operations quickly & efficiently simply by selecting each function individually.
Manual functions:
• Load tips
• Eject tips
• Aspirate reagents
• Dispense reagents
• Mix reagents
• Blow out reagents
Automated Program Mode: Create a protocol made up of multiple steps in succession. This interface allows for complex protocols to be run from tip-loading through completion with the touch of a button.
Programmable steps:
• Load/Eject Tips
• Aspirate/Dispense reagents
• Perform serial dilutions
• Rinse tips
• Repeat a cycle
• Pause after a certain step
Broad Labware Compatibility: All brands of microplates and reservoirs meeting SLAS/ANSI formats are compatible for use with the AutoMATE 96. Included plate adapters allow 384 well microplates and PCR plates to be used.
Fully Automated Tip Loading and Ejection: Motorized tip loading and ejection provides handsfree, consistent tip loading throughout all 96 wells.
Accuris Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. Accuris Taq Polymerase exhibits a 5′ to 3′ nuclease activity, but no 3′ to 5′ (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.
The polymerase is supplied with a 5X buffer containing MgCl2 and proprietary mix of enhancers (dNTP’s not included).
Laboratory balances, from economy to expensive analytical models, require routine calibration and accuracy confirmation. Accuris™ offers a selection of individual weights and sets that meet the standards for a variety of balance types.
We offer a variety of Class E2, F1, and M1 class weights, individually and in sets. All individual weights are supplied in protective, screw capped plastic containers, and weight sets are supplied in protective carrying cases.
Accuris Analytical balances incorporate electromagnetic sensor technology and precision mechanical components to offer superior weight determinations to a fraction of a milligram. Two capacity ranges are available in the Analytical Balance line to weigh samples up to 120g or 210g. Readability for both ranges is 0.0001g (0.1mg), with accuracy and linearity specifications that match up with the top brands in the industry. The W3100 balances tare and stabilize quickly to provide immediate results.
Both ranges offer an optional “Quick-Cal™” internal calibration feature that allows quick and convenient calibration without the need for a calibration standard weight. The glass draft shield enclosing the weighing chamber has sliding doors on the sides and top for easy access to the weigh pan. Leveling feet on the rear of the balance extend laterally to provide stability, and a sensitive leveling bubble aids in the leveling process.
The Accuris analytical balances have feature-rich software with different modes available for basic weighing in grams, ounces or carats, piece count weighing, determining percent deviation, and also check weighing for component inspection. The large, backlit LCD control panel is easy to see and easy to use.
The Accuris Series Dx Analytical Balances are robust, workhorse instruments that utilize Electromagnetic Force Restoration (EFR) technology. EFR is the gold standard for fast and reliable sub milligram mass determinations.
The waterproof and wear resistant front panel has been designed to hold up to spills and years of constant use. A brightly lit, dot matrix graphical display clearly shows weighing results and also enables an intuitive menu system that’s easy to work with. Useful features such as dynamic animal weighing, piece counting, percentage weighing and more are quickly accessible.
The front feet of the Dx balances are adjustable for proper leveling of the on a stable surface, and a leveling bubble behind the weighing chamber confirms proper installation.
A Quik-Cal™ calibration system helps ensure that the balance is always calibrated and ready for use. Simply press the CAL button to perform an automatic calibration using the internal precision calibration weight. Auto calibration can also be set to occur at specific time intervals depending on user preference.
Technical Data:
The Accuris E3200 UV Transilluminator offers a viewing surface of 21 x 26 cm that can accommodate larger gels or multiple small gels. High powered, 14-Watt UV bulbs provide higher sensitivity, so the smallest bands of stained nucleic acids can be visualized. This E3200 UV Transilluminator also features a control knob for continuous adjustment of the UV intensity.
The ASPIRE Laboratory Aspirator from Accuris features a quiet, maintenance-free pump inside a sturdy yet compact base. The vacuum pressure is fully adjustable, with an easy to read pressure gauge that displays the vacuum level at all times. The Polycarbonate collection bottle incorporates a reliable Overflow Protection Valve that closes off the tube to the vacuum once bottle is completely full. In addition, the replaceable in-line filter protects the vacuum pump from contaminants and any moisture that comes through the bottle. Graduations on the collection bottle indicate the amount of fluid collected and lets you know when it’s time to empty. The Handheld Vacuum Controller has an ergonomic design that’s comfortable for you during prolonged use. Pressing the button applies the vacuum. The pipette accepts Pasteur pipettes and serological pipettes. It also comes with 2 accessory pipette cones. One cone designed to accommodate 200/300ul pipette tips, the other has a thin, stainless steel capillary for fine control aspirating.
The ASPIRE aspirator is an excellent choice for laboratory aspirating applications, including removal of media from tissue culture vessels or plates, and supernatants. An ergonomic, hand held controller is included, and holds standard 200 to 300ul disposable pipette tips.
The SmartDrop X by Accuris is a full spectrum UV-Vis Spectrophotometer. The software has preset programs for BCA, Bradford and Lowry assays in addition to nucleic acid and protein calculations. Full spectrum analysis is completed in about 6 seconds, aiding in the identification of unknowns. Select up to 5 specific points on the spectrum for colorimetric readings output.
The SmartDrop L by Accuris determines nucleic acid and protein concentrations and purity of samples making it the ideal choice for laboratories. This spectrophotometer uses a LED light source and silicon protocell sensor to provide readings as 260 and 280nm. Samples ranging from 1.0 to 2.0ul are loaded directly on the pedestal providing results in about 5 seconds. Includes software that automatically calculates and displays 260/280 rations.
The SmartDrop XF by Accuris is not only a full spectrum UV-Vis Spectrophotometer, but also acts as a fluorometer. Features predefined programs for nucleic acids, proteins, and various colorimetric assays. The patented, motorized system gently stretches the sample column and allows for three different path lengths, providing accurate readings of samples from 0.5 to 2.0µl without dilution.
Cuvette 0D600 detection
Fluorescence detection
Accuris Precision balances quickly and accurately display sample measurements on a large, backlit LCD screen. Utilizing highly precise, gold plated ceramic capacitance sensors, these balances tare and stabilize quickly and provide reliable weight measurements within 0.001 grams.
All models feature a control panel with a large, bright back-lit LCD screen that is easy to use and easy to see. The advanced software includes weighing modes for standard weighing, piece counting, percent deviation and also check weighing for component inspection.
All Accuris Precision balances can be quickly calibrated in the lab with a few key strokes on the control panel and the use of a calibration weight. Leveling feet on the front are easy to access, and a leveling bubble integrated into the control panel aids in the leveling process
Technical Data:
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